KIFC3 Regulates the progression and metastasis of gastric cancer via Notch1 pathway

  • Yang He
    Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China

    Central Laboratory of Renmin Hospital, Wuhan, Hubei Province, China
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  • Pengzhan He
    Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China

    Central Laboratory of Renmin Hospital, Wuhan, Hubei Province, China
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  • Shimin Lu
    Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China

    Central Laboratory of Renmin Hospital, Wuhan, Hubei Province, China
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  • Weiguo Dong
    Corresponding author at: Department of Gastroenterology, Renmin Hospital of Wuhan University, No. 238 Zhang Zhi-dong Road, Wuhan 430060, Hubei Province, PR China.
    Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China

    Central Laboratory of Renmin Hospital, Wuhan, Hubei Province, China
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Published:March 06, 2023DOI:



      KIFC3 is a member of the kinesin family which has shown great promise in cancer therapy recently. In this study, we sought to elucidate the role of KIFC3 in the development of GC and its possible mechanisms.


      Two databases and a tissue microarray were used to explore the expression of KIFC3 and its correlation with patients’ clinicopathological characteristics. Cell proliferation was examined by cell counting kit-8 assay and colony formation assay. Wound healing assay and transwell assay were performed to examine cell metastasis ability. EMT and Notch signaling related proteins were detected by western blot. Additionally, a xenograft tumor model was established to investigate the function of KIFC3 in vivo.


      The expression of KIFC3 was upregulated in GC, and was associated with higher T stage and poor prognosis in GC patients. The proliferation and metastasis ability of GC cells were promoted by KIFC3 overexpression while inhibited by KIFC3 knockdown in vitro and in vivo. Furthermore, KIFC3 might activate the Notch1 pathway to facilitate the progression of GC, and DAPT, an inhibitor of Notch signaling, could reverse this effect.


      Together, our data revealed that KIFC3 could enhance the progression and metastasis of GC by activating the Notch1 pathway.



      GC (gastric cancer), KIFs (kinesin superfamily proteins), EMT (epithelial-mesenchymal transition), ADAM (an disintegrin and metalloproteinase domain-containing protein), TCGA (The Cancer Genome Atlas), IHC (immunohistochemistry), GSI (γ-secretase inhibitor)
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