Abstract
Background
Long non-coding RNAs (lncRNAs) have been implicated as functional molecules in hepatocellular
carcinoma (HCC) progression. The present research aimed to investigate the levels
of LncRNA cancer susceptibility candidate gene 19 (CASC19) in HCC tissues and cell
lines and to explore its potential role in the diagnosis and prognosis of HCC.
Methods
HCC tissues and cell lines were collected to assess the levels of CASC19 by real-time
quantitative reverse transcription PCR (RT-qPCR). The prognostic value of CASC19 was
evaluated using the Kaplan-Meier method and Cox regression analysis. The functional
role of CASC19 in regulating HCC cell proliferation, migration, and invasion was evaluated
by Cell Counting Kit-8 (CCK-8) and Transwell analysis. The potential targeted miR-140–5p
of CASC19 was confirmed by a dual-luciferase reporter assay.
Results
High CASC19 expression positively correlated with tumor size, differentiation, and
TNM stage in HCC patients (P < 0.05). Patients with high CASC19 expression have a poorer survival prognosis and
are prone to relapse compared to those with low CASC19. miR-140–5p, a target miRNA
for CASC19, negatively correlated with CASC19 levels in tumor tissues. Reduced CASC19
levels attenuated cell proliferation, migration, and invasion, but this attenuation
was reversed by suppression of miR-140–5p.
Conclusion
Up-regulated CASC19 may serve as a biomarker for predicting poor prognosis in HCC
patients. In vitro, overexpressed CASC19 promoted the progression of HCC, indicating
that CASC19 may be a possible therapeutic target for the treatment of HCC.
Keywords
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Article info
Publication history
Published online: February 17, 2023
Accepted:
December 4,
2022
Received:
August 3,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2022 Published by Elsevier Ltd on behalf of Editrice Gastroenterologica Italiana S.r.l.
