Abstract
Background and aims
Hepatocellular carcinoma (HCC) is one of the most common diseases threatening human
health worldwide. However, the molecular mechanisms of HCC are still unclear. Here,
we identified a differentially expressed lncRNA called MAPKAPK5-AS1(abbreviation:
MK5-AS1) and elucidated its role and molecular mechanism in the development of HCC.
Methods
Real-time PCR (RT-PCR) was used to verify the expression of MK5-AS1 in hepatocarcinoma
cell lines and tumor tissues of HCC patients. The biological functions of MK5-AS1
in HCC cells was assessed both in vitro and in vivo assays. The Lncbase, miRDB and
TargetScan databases were used to predict the lncRNA-miRNA-mRNA interactions. RNA
immunoprecipitation (RIP) and double luciferase reporter gene assays further verified
the interactions.
Results
MK5-AS1 expression was significantly upregulated in HCC tissues and cell lines. Furthermore,
high MK5-AS1 expression was positively associated with tumor progression and poor
prognosis. In vitro and in vivo experiments confirmed that overexpressed MK5-AS1 promoted
migration and invasion of HCC cells. Bioinformatics analysis based on Lncbase, miRDB
and TargetScan databases showed MK5-AS1 competitively bound to miR-376b-5p that prevented
epithelial cell transforming sequence 2 (ECT2) from miRNA-mediated degradation, thus
facilitated HCC metastasis.
Conclusion
Our results established a tumor promotive role of MK5-AS1 in HCC pathogenesis.
Keywords
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Article info
Publication history
Published online: December 23, 2022
Accepted:
November 30,
2022
Received:
July 9,
2022
Editor: Roberto de FranchisPublication stage
In Press Corrected ProofIdentification
Copyright
© 2022 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
