Association between multiple gene promoter hypermethylation and the risk of gastric cancer: A systematic review and meta-analysis

  • JianRu Wen
    College of Medicine, Southwest Jiaotong University, Chengdu 610031, China

    Department of Gastroenterology, The General Hospital of Western Theater Command, Chengdu 610083, China
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  • SiXiu Deng
    Department of Gastroenterology, The General Hospital of Western Theater Command, Chengdu 610083, China

    College of Medicine, Southwest Medical University, Luzhou 646000, China
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  • YuHua Bi
    College of Medicine, Southwest Jiaotong University, Chengdu 610031, China

    Department of Gastroenterology, The General Hospital of Western Theater Command, Chengdu 610083, China
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  • LiJuan Qiao
    Department of Gastroenterology, The General Hospital of Western Theater Command, Chengdu 610083, China
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  • Hui Xu
    Corresponding author at: College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.
    College of Medicine, Southwest Jiaotong University, Chengdu 610031, China

    Department of Gastroenterology, The General Hospital of Western Theater Command, Chengdu 610083, China
    Search for articles by this author
Published:April 18, 2022DOI:



      Several studies have demonstrated an association between multiple gene hypermethylation and gastric cancer. However, the intrinsic mechanisms remain elusive and highly debatable. To this end, our study aims to investigate the correlation between the methylation status of multiple gene promoters and gastric cancer.


      PubMed, EMBASE, CNKI, WanFang, Cqvip, and Cochrane Library were queried from inception to May 2021, and the relationship between the methylation status of the CpG islands and gastric cancer risk was systematically assessed under the inclusion and exclusion criteria. The incidence of DNA methylation between tumor and non-tumor tissues was compared, and the clinicopathological significance of DNA methylation in gastric carcinoma was further evaluated. The odds ratio (OR) was estimated with a 95% confidence interval (CI), and forest plots were generated using the fixed-effects or random-effects model.


      In total, 201 studies were enrolled, and a higher frequency of CpG islands methylation was identified in gastric cancer tissues than in non-neoplastic tissues. This suggests that aberrant polygene methylation might be associated with the initial onset and progression of gastric cancer.


      This study sheds light on the significance of polygene methylation status in gastric cancer. The DNA methylation of these genes may serve as underlying epigenetic biomarkers, providing a promising molecular diagnostic approach for human gastric cancer clinical diagnosis. More large randomized trials are needed to confirm the findings.


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