Abstract
Background
Diagnosis of malignant biliary obstruction is complicated and lacks accuracy. Exosomes
may be secreted by malignant tumors; intact miRNAs from exosomes might serve as potential
biomarkers for the disease.
Aim
To identify exosomal microRNAs in human bile among benign and malignant biliary obstructions.
Methods
Bile samples were collected from patients undergoing therapeutic endoscopic retrograde
cholangiopancreatography for biliary obstruction. Exosome microRNAs were determined
by RNA-sequencing in the discovery cohort, which comprising benign (n = 5) cases and
malignant biliary obstruction (n = 5) cases. Then, the diagnostic performance of the
two up-regulated microRNAs (mir-483-5p and mir-126-3p) of bile exosomes was verified
by analysis of 82 patients with a diagnosis of malignant (n=37) or nonmalignant (n=45)
biliary obstruction.
Results
In both cohorts, the expressions of mir-483-5p and mir-126-3p were significantly higher
in bile exosomes samples from patients with malignant biliary obstructions than controls.
In the verification cohort, the two miRNAs can distinguished the benign and malignant
groups with high diagnostic accuracy and specificity; the diagnostic values of the
two microRNAs were better than serum carbohydrate antigen 19-9 (CA19-9), area under
the curve (AUC) were 0.81 and 0.74.
Conclusion
The expression of exosomal mir-483-5p and mir-126-3p in the bile samples discriminates
between patients with malignant and nonmalignant biliary obstructions.
Clinical Trial registration no
NCT03102268
Keywords
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Article info
Publication history
Published online: November 27, 2020
Accepted:
November 9,
2020
Received:
April 25,
2020
Identification
Copyright
© 2020 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
