Abstract
Background
No study has compared the performance of light microscopy (LM) and whole slide imaging
(WSI) for endoscopic ultrasound (EUS) histological acquired tissue samples from pancreatic
solid lesions (PSLs). We evaluated the concordance between LM and WSI and the inter-
and intra-observer agreements among pathologists on PSLs EUS acquired samples.
Methods
LM and WSI from 60 patients with PSLs were evaluated by five expert pathologists to
define: diagnostic classification, presence of a core, number and percentage of lesional
cells. Washout period between evaluations was 3 months. Time of the procedures was
also assessed.
Results
Forty-eight cell-block and 12 biopsy samples were evaluated. A high concordance between
LM and WSI was found. Inter- and intra-observer agreements for diagnostic classification
were substantial and complete, respectively. For all the other parameters, the inter-observer
agreement was usually higher for LM. For the intra-observer, a substantial agreement
was reached regarding the presence of tissue core and the number and the percentage
of malignant cells. Median time for performing LM was significantly shorter than for
WSI (p < 0.0001).
Conclusions
LM and WSI of cell-block and biopsy samples acquired by EUS in PSLs were highly concordant,
with a substantial inter-observer and a complete intra-observer agreements regarding
diagnostic classification.
Keywords
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Article info
Publication history
Published online: May 28, 2019
Accepted:
April 29,
2019
Received:
March 10,
2019
Identification
Copyright
© 2019 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
