Abstract
Background
Many studies have reported significant changes in intestinal microbiota in irritable
bowel syndrome (IBS) patients based on quantitative real-time PCR analysis.
Aims
We aimed to review the alterations in intestinal microbiota.
Methods
An online search up to June 9, 2016, was conducted. This systematic review and meta-analysis
included differential expression of intestinal microbiota in patients with IBS versus
healthy controls (HCs) and subgroup analysis. We assessed the quality of the included
studies using an original assessment tool.
Results
A total of 13 articles involving 360 IBS patients and 268 healthy controls were included.
The quality assessment scores for these articles ranged from 5 to 8. Significant differences
in expression in IBS patients were observed for Lactobacillus (SMD = −0.85, P < 0.001, I2 = 28%), Bifidobacterium (SMD = −1.17, P < 0.001, I2 = 79.3%), and Faecalibacterium prausnitzii (SMD = −1.05, P < 0.001, I2 = 0.0%) but not Bacteroides-Prevotella group, Escherichia coli or other genera or species. Subgroup analysis showed that diarrhea-predominant IBS
patients had significantly different expression of Lactobacillus (SMD = −1.81, P < 0.001) and Bifidobacterium (SMD = −1.45, P < 0.001).
Conclusion
Down-regulation of bacterial colonization including Lactobacillus, Bifidobacterium and F. prausnitzii was observed in IBS patients, particularly in diarrhea-predominant IBS (IBS-D). Microbiota
changes participate in the pathogenesis of IBS and may underlie the efficacy of probiotic
supplements.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Digestive and Liver DiseaseAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Functional bowel disorders.Gastroenterology. 2006; 130: 1480-1491
- Harrison’s principles of internal medicine.16th ed. McGraw-Hill, New York2004
- Review article: evidence for the role of gut microbiota in irritable bowel syndrome and its potential influence on therapeutic targets.Alimentary Pharmacology and Therapeutics. 2014; 39: 1033-1042
- Irritable bowel syndrome and small intestinal bacterial overgrowth: meaningful association or unnecessary hype.World Journal of Gastroenterology. 2014; 20: 2482-2491
- Methodological issues in the study of intestinal microbiota in irritable bowel syndrome.World Journal of Gastroenterology. 2014; 20: 8821-8836
- The STARD statement for reporting studies of diagnostic accuracy: explanation and elaboration.Clinical Chemistry. 2003; 49: 7-18
- Differential expression of intestinal microbiota in colorectal cancer compared with healthy controls: a systematic review and meta-analysis.International Journal of Clinical and Experimental Medicine. 2016; 9: 10923-10930
- Changes of intestinal microflora in children with irritable bowel syndrome by SYBR Green I Real Time Fluo-rescent Quantitative PCR Method.Medical and Pharmaceutical Journal of Chinese People’s Liberation Army. 2014; 26: 93-96
- Analysis of patients with intestinal target bacteria diarrhea type irritable bowel syndrome.Acta Universitatis Medicinalis Anhui. 2014; 49: 653-658
- Increase in fecal primary bile acids and dysbiosis in patients with diarrhea-predominant irritable bowel syndrome.Neurogastroenterology and Motility. 2012; 24 (e246-7): 513-520
- Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome.Neurogastroenterology and Motility. 2010; 22 (e114-5): 512-519
- Analysis of the fecal microbiota of irritable bowel syndrome patients with 16S rDNA real-time PCR.Shandong Medical Journal. 2009; 49: 51-52
- Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR.The American Journal of Gastroenterology. 2005; 100: 373-382
- Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR.Journal of Applied Microbiology. 2004; 97: 1166-1177
- Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis.Applied and Environmental Microbiology. 2001; 67: 2578-2585
- Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR.FEMS Microbiology Ecology. 2009; 68: 351-362
- Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces.Applied and Environmental Microbiology. 2002; 68: 5445-5451
- Comparison of real-time PCR with SYBR Green I or 5′-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria.Microbiology. 2003; 149: 269-277
- Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR.Journal of Clinical Microbiology. 2002; 40: 4423-4427
- Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients.Proceedings of the National Academy of Sciences of the United States of America. 2008; 105: 16731-16736
- Fecal microbiota in patients with irritable bowel syndrome compared with healthy controls using real-time polymerase chain reaction: an evidence of dysbiosis.Digestive Diseases and Sciences. 2015; 60: 2953-2962
- Molecular analysis of faecal and duodenal samples reveals significantly higher prevalence and numbers of Pseudomonas aeruginosa in irritable bowel syndrome.Journal of Medical Microbiology. 2011; 60: 236-245
- Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome.Gut Pathogens. 2010; 2: 19
- Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers.BMC Gastroenterology. 2009; 9: 95
- Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification.World Journal of Gastroenterology. 2009; 15: 5936-5945
- Lower bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients.World Journal of Gastroenterology. 2009; 15: 2887-2892
- Diversity of the human intestinal microbial flora.Science. 2005; 308: 1635-1638
- Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome.Neurogastroenterology and Motility. 2012; 24: 31-39
- Gastrointestinal microbiome signatures of pediatric patients with irritable bowel syndrome.Gastroenterology. 2011; 141: 1782-1791
- Next-generation DNA sequencing.Nature Biotechnology. 2008; 26: 1135-1145
- An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota.Gut. 2012; 61: 997-1006
- Altered faecal and mucosal microbial composition in post-infectious irritable bowel syndrome patients correlates with mucosal lymphocyte phenotypes and psychological distress.Alimentary Pharmacology and Therapeutics. 2015; 41: 342-351
- IBS-associated phylogenetic unbalances of the intestinal microbiota are not reverted by probiotic supplementation.Gut Microbes. 2012; 3: 406-413
- Contribution of the microflora to proteolysis in the human large intestine.Journal of Applied Bacteriology. 1988; 64: 37-46
- Activation of human enteric neurons by supernatants of colonic biopsy specimens from patients with irritable bowel syndrome.Gastroenterology. 2009; 137: 1425-1434
- A serpin from the gut bacterium Bifidobacterium longum inhibits eukaryotic elastase-like serine proteases.The Journal of Biological Chemistry. 2006; 281: 17246-17252
- Lactulose breath testing does not discriminate patients with irritable bowel syndrome from healthy controls.The American Journal of Gastroenterology. 2008; 103: 958-963
- Evaluating breath methane as a diagnostic test for constipation-predominant IBS.Digestive Diseases and Sciences. 2010; 55: 398-403
- Pseudomonas aeruginosa as a cause of infectious diarrhoea.Epidemiology and Infection. 1998; 121: 237-241
- Pseudomonas aeruginosa elastase disables proteinase-activated receptor 2 in respiratory epithelial cells.American Journal of Respiratory Cell and Molecular Biology. 2005; 32: 411-419
- Clinical relevance of proteinase activated receptors (pars) in the gut.Gut. 2005; 54: 867-874
Article info
Publication history
Published online: January 21, 2017
Accepted:
January 9,
2017
Received:
June 19,
2016
Identification
Copyright
© 2017 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
